Experimental Protocal: Colon Cancer Study
To test the hypothesis (given in the previous slide), we used the following experimental protocol. Five hundred and sixty male Sprague Dawley rats were allocated to a 2 by 2 by 2 by 3 factorial design with two sources of lipid (corn oil or fish oil), two types of fiber (pectin or cellulose), with or without irradiation (1 Gray iron ions, at Brookhaven National Laboratory), and terminated at three different time points–the initiation stage, the promotion stage and at the final tumor endpoint. Fecal material was collected throughout the study from a subset of rats, and mRNA was analyzed for changes in gene expression profiles using microarray technology.
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