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Light Microscopy: Comparison of Optics

Light Microscopy: Comparison of Optics

Darkfield x400 (left) and brightfield x2000.
© Micrographia\John Walsh.

Scientists use a variety of methods to view different subjects through a light microscope. Dr. David Caprette explains different optics (bright field, dark field, phase contrast, etc.) and the some of the best uses for each.

This slide set accompanies the presentation, "Light Microscopy: Comparison of Optics."

Author(s): David R. Caprette, PhD
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Compound Light Microscope

Refraction

Magnification and Orientation I

Magnification and Orientation II

Resolution

“Empty” Magnification

Depth of Focus

Field of View and Light Intensity

Contrast

Magnifying and Observing Cells

What Are These?

Typical Parts of Plant Cells

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