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Light Microscopy: Comparison of Optics

Light Microscopy: Comparison of Optics

Darkfield x400 (left) and brightfield x2000.
© Micrographia\John Walsh.

Scientists use a variety of methods to view different subjects through a light microscope. Dr. David Caprette explains different optics (bright field, dark field, phase contrast, etc.) and the some of the best uses for each.

This slide set accompanies the presentation, "Light Microscopy: Comparison of Optics."

Author(s): David R. Caprette, PhD
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Preparing Wet Mount Slides of Plant Cells

Making a Wet Mount Slide

Comparing Onion and Elodea Cells

Most Organisms are Single-Celled

Measuring and Counting with a Light Microscope

Stage Micrometer

Estimating Field Diameter of a Microscope

Estimating Number of Objects Per Unit Area

Calibrating an Eyepiece Reticule

Measuring with an Eyepiece Reticule

Resolving and Reporting Dimensions

Calibrating the Fine Focus Control

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