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Light Microscopy: Comparison of Optics

Light Microscopy: Comparison of Optics

Darkfield x400 (left) and brightfield x2000.
© Micrographia\John Walsh.

Scientists use a variety of methods to view different subjects through a light microscope. Dr. David Caprette explains different optics (bright field, dark field, phase contrast, etc.) and the some of the best uses for each.

This slide set accompanies the presentation, "Light Microscopy: Comparison of Optics."

Author(s): David R. Caprette, PhD
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Using the Fine Focus to Measure Depth

Using Volume to Estimate Depth

Counting Chamber (Hemacytometer)

Using a Hemacytometer

Example: Counting a Cell Suspension

Example: Calculating a Cell Concentration

Sessions 2-4: Follow-up Observations

Extension

Observing Different Microbes

Engage Students by Asking...

Prepare Slides of Microbes to Observe

Let's Talk About Bacteria

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