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Measuring and Counting with a Light Microscope

Measuring and Counting with a Light Microscope

Learn how to calibrate a light microscope for measurement, how to estimate field diameter, and how to count the number of objects in a single field.

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Author(s): David R. Caprette, PhD
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Measuring and Counting with a Light Microscope

Stage Micrometer

Estimating Field Diameter of a Microscope

Estimating Number of Objects Per Unit Area

Calibrating an Eyepiece Reticule

Measuring with an Eyepiece Reticule

Resolving and Reporting Dimensions

Calibrating the Fine Focus Control

Using the Fine Focus to Measure Depth

Using Volume to Estimate Depth

Counting Chamber (Hemacytometer)

Using a Hemacytometer

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