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Using a Bright Field Light Microscope

Author(s): David R. Caprette, PhD

Using a Bright Field Light Microscope

This presentation will take us through the steps required to view a specimen in a bright field light microscope. We will cover proper mounting of a specimen, adjusting the condenser and oculars, and strategies for finding a target and for working up in magnification. We will discuss how the thickness of a specimen can limit the range of useful magnifications, and how to use oil immersion objective lenses to obtain the best resolution possible in a bright field microscope.