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Using a Micropipettor

Author(s): Sonia Rahmati Clayton, PhD

Micropipettor: Loading the Tip

Once the volume is set and the disposable tip is firmly placed on the shaft, you can begin the liquid transfer process. Depress the plunger button to the first resistance point and hold. Next, immerse the disposable tip into the liquid sample. Be careful only  to immerse the tip and not the shaft of the micropipettor into the sample. Gently release the plunger drawing fluid into the tip. It is important to do this slowly, or you will create bubbles and "splash" the liquid inside the tip. Splashing contaminates the sample, the shaft and the piston space inside the shaft.